Is your bovine serum albumin (BSA) chosen correctly?

Is your bovine serum albumin (BSA) chosen correctly?

In the process of reagent development, we will use BSA more or less to promote stable performance and strong specificity of the reagent. Since bovine serum albumin (BSA) has a variety of product styles, it can meet different applications. At present, most of the information on the Internet is ambiguous. There are bovine serum albumin and bovine serum albumin v component, etc. Many suppliers are not clear about the difference; in addition, the market also provides protease-free, DNase-free and RNase-free, There are no fatty acid, microbial grade, reagent grade, diagnostic analysis grade and other BSA powders, so how to choose a suitable BSA product is an important prerequisite. Today, I will take you to see the difference in usage of different levels of BSA.

We know that ordinary BSA powder or the fifth component is suitable for cell and tissue culture, and can also be used for some protein supplements; while other BSA without protease, DNase, RNase, fatty acid, etc. are suitable for more Immunology, proteology and other applications, as a blocking agent, protein marker, hapten carrier and protein stabilizer. The editor will give a detailed introduction below:

Standard Grade BSA

Common BSA products that have been heat-treated can be used as culture nutrients and ingredients for tissue cells (microorganisms, animal and insect cells, etc.) and protein molecular weight standard components. They are cost-effective BSA products, such as EquiTech-Bio's BAH62 series products.

BSA fifth component

The fifth component of BSA is more pure than ordinary BSA. The fifth component of BSA is purified by the Cohn method. It is a biotechnological grade product that can generally be used for cell culture, and can also be used as a hapten carrier, protective agent, and protein supplement control. Such as EquiTech-Bio's BAC62 or BAC63 series products.

IgG free reagent grade BSA

Reagent grade BSA has better purity than BSA 5th fraction, and the powder does not contain globulin IgG components that may interfere with the analysis. It can be used as tissue cell culture nutrient and culture components and protein molecular weight standard components. Such as EquiTech-Bio's BAH64 series products.

protease-free BSA

Protease-free bovine serum albumin can be used in cell culture protein production experiments, especially for expressing proteins that are sensitive to protease activity. In addition, protease-free bovine serum albumin is also very suitable. Such as EquiTech-Bio's BAH67 series of BSA products and BAC65 series of fifth component products.

Fatty Acid Free BSA

Fatty acid-free BSA can be used as a carrier protein for fat metabolism-related analysis, and can also be combined with a specific fatty acid to form albumin, which can be used to enhance the performance of cell culture systems; if the presence of fat or fatty acids in the experiment affects hormones or cholesterol This product is the first choice when it comes to readings. Such as EquiTech-Bio's BAH66 series.

Low Endotoxin BSA

Low endotoxin bovine serum albumin is ideal for cell and tissue culture. It can be used as a carrier of nutritional supplements for serum-free and synthetic media, and as a general reagent in the laboratory to stabilize and dilute sensitive protein solutions. It can also be used as a diagnostic and analytical tool, such as ELISA. Such as EquiTech-Bio's BAH series, the endotoxin content is lower than 1.0 EU/mg.

Protease/IgG BSA

It does not contain protease and RNase enzyme activity, and does not contain IgG at the same time, with a higher purity level. It is used as a diluent or blocking solution in EIA and ELISA tests. Such as Jackson's 001-000-16X series and EquiTech-Bio's BAH67 (protease/IgG-free, RNase-free) series of products.

Diagnostic Grade BSA

Diagnostic grade bovine serum albumin-powder is specially designed to reduce factors that may cause background interference and cell culture system inhibition, does not contain proteases, bioburden and IgG, and has very low activity of endotoxin (less than 3.0 EU/mg) . It can be used for protein standards, diluents, conjugates or enzyme stabilizers, and can also be used for high-sensitivity immunoassay experiments, cell culture and hybridization experiments, etc. You can contact our operation to purchase diagnostic grade BSA.